Article 14221

Title of the article

The study of cell content dynamics depending on localization in the dermis after local cold injuries 

Authors

Nikita A. Shutskiy, Junior researcher, Central Scientific and Research Labaratory, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia) E-mail: nikitashutskijj@rambler.ru
Sergey L. Kashutin, Doctor of medical sciences, associate professor, head of the sub-department of skin and venereal deseases, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: sergeycash@yandex.ru
Leonid L. Shagrov, Junior researcher, Central Scientific and Research Labaratory, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: leonidshagrov@mail.ru
Lyubov' N. Gorbatova, Doctor of medical sciences, rector of Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: info@nsmu.ru
Svetlana I. Malyavskaya, Doctor of medical sciences, professor, vice-rector for research and innovation, head of the sub-department of pediatrics, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: nordnauka@yandex.ru
Oleg V. Kalmin, Doctor of medical sciences, professor, head of the sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: kalmin_ov@pnzgu.ru
Ivan M. Kobelev, Student, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: ivan_kobelev_2017@mail.ru 

Index UDK

616-092 

DOI

10.21685/2072-3032-2021-2-14 

Abstract

Background. There is a lot of literature on the study of the regeneration of skin components in thermal injuries. One of the important issues in understanding the recovery processes is the localization and time of accumulation of the main producers of the extracellular matrix - fibroblasts, information about which is currently insufficient. The aim of this work is to study the dynamics of cellular structures, migratory injuries after local skin frostbite.
Materials and methods. The research was carried out on white outbred rats kept in the same conditions. After the onset of narcotic sleep, the animals were simulated local cold injury, dividing them into groups of 20 animals. After slaughter on days 3, 7, 14 and 21, biopsies of damaged skin and periphery were analyzed using conventional histological methods, and the number of cells in the zones of the papillary, reticular dermis and at the border of the reticular dermis and hypodermis was also counted.
Results. The modeling of local cold injury of the 3rd degree was carried out, the damaged skin integuments and areas located in the immediate vicinity of them were examined for the presence of cellular structures. The dynamics was revealed throughout the entire observation period.
Conclusions. Based on the data obtained, it was found that from 3 to 7 days, at the border of the reticular dermis and hypodermis, not only an increase in cell accumulation in the area of the damaged area was noted, but also directly in the intact skin located next to the lesion focus. Since the content of cells per unit area is greater precisely at the border of the reticular dermis and hypodermis, it can be assumed that the formation of collagen occurs largely in this area than in the papillary or reticular dermis. 

Key words

cells, fibroblasts, local cold injury, dermis 

Download PDF
References

1. Durymanov M.O., Boyarintsev V.V., Biryukov S.A., Gorina E.V., Filkov G.I. Methodological Approaches to Development of Cell-based Medicinal Product for Treatment of Patients with Cold Injury in the Arctic. Hum Physiol. 2020;46:798–805.
2. Chabanenko O.O., Kovalov G.O., Iefimova I.A. Effect of Local Cold Injury on Osmotic Resistance of Erythrocytes. Problems of Cryobiology and Cryomedicine. 2019;29(2):168–168.
3. Norrbrand L., Kölegård R., Keramidas M.E., Mekjavic I.B., Eiken O. Finger-and toetemperature responses to local cooling and rewarming have limited predictive value identifying susceptibility to local cold injury-a cohort study in military cadets. Applied ergonomics. 2020;82:102964.
4. Gurtner G.C., Werner S., Barrandon Y., Longaker M.T. Wound repair and regeneration. Nature. 2008;453(7193):314–321.
5. Desjardins-Park H.E., Foster D.S., Longaker M.T. Fibroblasts and wound healing: an update. Regenerative medicine. 2018;13(5):491–495.
6. Shutskiy N.A., Shagrov L.L., Kashutin S.L., Aksenov A.S., Malyavskaya S.I. Dynamics of collagen content in rat dermis after local cold injury. Trudy Karel'skogo nauchnogo tsentra Rossiyskoy akademii nauk = Proceedings of Karelia Scientific Center of the Russian Academy of Sciences. 2019;6:85–91. (In Russ.)
7. Lazarenko V.A., Artyushkova E.B., Mishustin V.N., Goryainova G.N., Monovtsov I.A., Kvachakhiya L.L., Chigunadze A.L. Pharmacological correction of morphological changes in experimental frostbite. Uchenye zapiski Orlovskogo gosudarstvennogo universiteta. Ser.: Estestvennye, tekhnicheskie i meditsinskie nauki = Proceedings of Orel State University. Series: Natural, engineering and medical sciences. 2013;6:218–227. (In Russ.)
8. Boyko V.V., Milovidova A.E., Yanovskaya L.G., Brusnitsyna M.P., Isaev Yu.I., Logachev V.K., Leont'eva L.V. The study of morphological features in tissues of experimental animals when modeling cold injury. Vestnik morfologii = Morphology bulletin. 2010;16(3):526–528. (In Russ.)
9. Korzhevskiy D.E. (ed.). Morfologicheskaya diagnostika. Podgotovka materiala dlya gistologicheskogo issledovaniya i elektronnoy mikroskopii = Morphological diagnostics. Preparation of material for histological examination and electron microscopy. Saint-Petersburg: SpetsLit., 2017:160. (In Russ.)
10. Avtandilov G.G. Osnovy kolichestvennoy patologicheskoy anatomii: ucheb. posobie. – Fundamentals of quantitative pathological anatomy: teaching aid. M., 2002. Arkhiv patologii. 2004;66(3):240. (In Russ.)

 

Дата создания: 24.08.2021 15:03
Дата обновления: 27.08.2021 12:41