Nikita A. Shutskiy, Junior researcher, Central Scientific and Research Labaratory, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia) E-mail: firstname.lastname@example.org
Sergey L. Kashutin, Doctor of medical sciences, associate professor, head of the sub-department of skin and venereal deseases, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: email@example.com
Leonid L. Shagrov, Junior researcher, Central Scientific and Research Labaratory, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: firstname.lastname@example.org
Lyubov' N. Gorbatova, Doctor of medical sciences, rector of Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: email@example.com
Svetlana I. Malyavskaya, Doctor of medical sciences, professor, vice-rector for research and innovation, head of the sub-department of pediatrics, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: firstname.lastname@example.org
Oleg V. Kalmin, Doctor of medical sciences, professor, head of the sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: email@example.com
Ivan M. Kobelev, Student, Northern State Medical University (51 Troitsky avenue, Arkhangelsk, Russia), E-mail: firstname.lastname@example.org
Background. There is a lot of literature on the study of the regeneration of skin components in thermal injuries. One of the important issues in understanding the recovery processes is the localization and time of accumulation of the main producers of the extracellular matrix - fibroblasts, information about which is currently insufficient. The aim of this work is to study the dynamics of cellular structures, migratory injuries after local skin frostbite.
Materials and methods. The research was carried out on white outbred rats kept in the same conditions. After the onset of narcotic sleep, the animals were simulated local cold injury, dividing them into groups of 20 animals. After slaughter on days 3, 7, 14 and 21, biopsies of damaged skin and periphery were analyzed using conventional histological methods, and the number of cells in the zones of the papillary, reticular dermis and at the border of the reticular dermis and hypodermis was also counted.
Results. The modeling of local cold injury of the 3rd degree was carried out, the damaged skin integuments and areas located in the immediate vicinity of them were examined for the presence of cellular structures. The dynamics was revealed throughout the entire observation period.
Conclusions. Based on the data obtained, it was found that from 3 to 7 days, at the border of the reticular dermis and hypodermis, not only an increase in cell accumulation in the area of the damaged area was noted, but also directly in the intact skin located next to the lesion focus. Since the content of cells per unit area is greater precisely at the border of the reticular dermis and hypodermis, it can be assumed that the formation of collagen occurs largely in this area than in the papillary or reticular dermis.
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